Catalog Number - 64424-10P

Nova Similars Phenyl™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped phenyl bonded phase. The stationary phase consists of a four-carbon linker terminating in a phenyl functional group, providing unique chromatographic selectivity through a combination of hydrophobic and π-π interactions. Designed as a cost-effective alternative to Waters™ Nova-Pak® Phenyl columns, it offers comparable chromatographic behavior for USP L11 methods while delivering the reproducibility and reliability required for routine analytical applications.

64424-10P is a 100 mm x 4.6 mm ID analytical column packed with 4 µm Nova Similars Phenyl particles. The 100 mm length provides an excellent balance between analysis speed and chromatographic resolution, making it suitable for routine testing, method development, and quality control applications. The 4 µm particle size provides improved efficiency and sharper peak shapes than comparable 5 µm columns while maintaining moderate operating pressure on conventional HPLC systems. Typical operating flow rates are approximately 1.0–2.0 mL/min.

This USP L11 column is particularly useful for aromatic compounds where phenyl selectivity can provide different retention and separation characteristics than traditional C18 phases. Common applications include pharmaceutical analysis, aromatic hydrocarbons, environmental testing, and methods requiring enhanced aromatic selectivity through π-π interactions.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L11 methods

• Comparable selectivity to Waters™ Nova-Pak® Phenyl columns

• Fully endcapped phenyl bonded phase

• Enhanced aromatic compound selectivity

• Unique π-π interaction mechanism

• Higher efficiency than comparable 5 µm columns

• Balanced speed and resolution with 100 mm length

Typical Applications

• USP L11 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Aromatic compound separations

• Environmental testing

• Method development

• Quality control testing

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Nova Similars Phenyl

• USP Classification: L11

• Particle Size: 4 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: Phenyl

• Endcapped: Fully

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Phenyl selectivity provides different retention than C18 phases

• Enhanced interactions with aromatic compounds

• 100 mm length balances speed and resolution

• Higher efficiency than comparable 5 µm columns

• Moderate operating pressure on conventional HPLC systems

• Excellent alternative for many USP L11 methods

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